List of validated methods

Bioanalytical method development and validation is carried out according FDA and EMA requirements. Highly sensitive analytical method can be developed in accordance with clinical protocol requirements.

The list of validated methods:

Compound (Metabolite, Biomarker)	Matrix	Method	LLOQ	Status^*
Abacavir	Plasma	LC-MS/MS	50 ng/mL	Active
Abacavir/ Lamivudine	Plasma	LC-MS/MS	50 ng/mL 50 ng/mL	Active
Abiraterone	Plasma	LC-MS/MS	0.2 ng/mL	Active
Acetaldehyde	Plasma	LC-MS/MS	10 ng/mL	Active
Acetaminophen / Chlorpheniramine	Plasma	LC-MS/MS	10 ng/mL 0.1 ng/mL	Active
Acetaminophen / Caffeine	Plasma	LC-MS/MS	10 ng/mL 100 ng/mL	Active
Acetylsalicylic acid / Salicylic acid	Plasma	LC-MS/MS	4 ng/mL 15 ng/mL	Active
Acotiamide	Plasma	LC-MS/MS	0.5 ng/ml	Active
Ademethionine	Plasma	LC-MS/MS	10 ng/mL	Active
Ambroxol	Plasma	LC-MS/MS	1 ng/mL	Active
Ambroxol / Cetirizine	Plasma	LC-MS/MS	1 ng/mL 4 ng/mL	Active
p-Aminosalicylic acid	Plasma	LC-MS/MS	50 ng/mL	Active
Amlodipine	Plasma	LC-MS/MS	0.2 ng/mL	Active
Amlodipine / Metoprolol	Plasma	LC-MS/MS	0.2 ng/mL 0.1 ng/mL	Active
Amlodipine / Telmisartan	Plasma	LC-MS/MS	0.2 ng/mL 2.5 ng/mL	Active
Amoxicilline / Clavulanic acid	Plasma	LC-MS/MS	100 ng/mL 50 ng/mL	Active
Anagrelide	Plasma	LC-MS/MS	50 pg/mL	Active
Anandamide	Plasma	LC-MS/MS	0.1 ng/mL	Active
2-Arachidonoyl glycerol	Plasma	LC-MS/MS	0.5 ng/mL	Active
Arginine	Plasma	LC-MS/MS	4 µg/mL	Active
Atazanavir	Plasma	LC-MS/MS	5 ng/mL	Active
Atorvastatin	Plasma	LC-MS/MS	0.2 ng/mL	Active
Atorvastatin / Valsartan	Plasma	LC-MS/MS	0.2 ng/mL 10 ng/ml	Active
Azacitidine	Plasma	LC-MS/MS	3 ng/ml	Active
Bosentan	Plasma	LC-MS/MS	5 ng/mL	Active
Bicalutamide	Plasma	LC-MS/MS	10 ng/mL	Active
Bisoprolol	Plasma	LC-MS/MS	0.5 ng/mL	Active
Buspirone / 6-Hydroxy buspirone / 2-(1-Piperazinyl)pyrimidine (1-PP)	Plasma	LC-MS/MS	20/50pg/mL 0.5 ng/mL	Active
Capecitabine / 5-Fluorouracil	Plasma	LC-MS/MS	20 ng/mL 20 ng/mL	Active
Celecoxib	Plasma	LC-MS/MS	10 ng/mL	Active
Cetirizine (Levocetirizine)	Plasma	LC-MS/MS	1 ng/mL	Active
Chlorpromazine	Plasma	LC-MS/MS	1 ng/mL	Active
Choline	Plasma	LC-MS/MS	50 ng/mL	Active
Cinacalcet	Plasma	LC-MS/MS	0.1 ng/mL 0.05 ng/mL	Active
Cisplatine	Plasma	LC-MS/MS	1 ng/mL	Active
Citalopram	Plasma	LC-MS/MS	0.3 ng/mL	Active
Clopidogrel / Carboxylic acid Clopidogrel	Plasma	LC-MS/MS	10 pg/ml 100 ng/mL	Active
Coenzyme Q₁₀/ reduced Coenzyme Q₁₀(ubiquinol)/ Coenzyme Q₉	Plasma Tissue	LC-MS/MS	1 µg/mL	Active
Corticosterone	Urine	LC-MS/MS	5 ng/mL	Active
Cortineff	Urine	LC-MS/MS	1 ng/mL	Inactive
Cortisol (6ß-Hydroxycortisol)	Urine	LC-MS/MS	5 ng/mL	Active
Cortisone	Urine	LC-MS/MS	5 ng/mL	Active
Creamide	Plasma	LC-MS/MS	0.1 µg/mL	Active
Cycloserine	Plasma	LC-MS/MS	0.3 µg/mL	Active
Dabigatran free/total	Plasma	LC-MS/MS	0.5 ng/mL	Active
Dapoxetine	Plasma	LC-MS/MS	1 ng/mL	Active
Darunavir	Plasma	LC-MS/MS	50 ng/mL	Active
Dasatinib	Plasma	LC-MS/MS	1 ng/mL	Active
Desloratadin	Plasma	LC-MS/MS	50 pg/ml	Active
Dexamethasone	Urine	LC-MS/MS	1 ng/mL	Active
Dexketoprofen	Plasma	LC-MS/MS	50 ng/mL	Active
Diclofenac / Tolperisone	Plasma	LC-MS/MS	50 ng/mL 0.5 ng/mL	Active
Docetaxel	Plasma	LC-MS/MS	2 ng/mL	Active
Dopamine	Plasma Urine	HPLC/ED	0.35 ng/mL	Active
Doxazosin	Plasma	LC-MS/MS	0.3 ng/mL	Active
Doxepin / N-Desmethyldoxepin	Plasma	LC-MS/MS	100 pg/ml 100 pg/ml	Active
Doxorubicin	Plasma Tissue	LC-MS/MS	0.5 ng/mL	Active
Doxylamine	Plasma	LC-MS/MS	0.2 ng/mL	Active
Drospirenone	Plasma	LC-MS/MS	0.2 ng/mL	Active
Dutasteride / Tamsulosin	Plasma	LC-MS/MS	0.1/ 0.2 ng/mL	Active
Ebastine Carebastin	Plasma	LC-MS/MS		Development
Efavirenz	Plasma	LC-MS/MS	100 ng/mL	Active
Emtricitabine	Plasma	LC-MS/MS	20 ng/mL	Active
Emtricitabine / Tenofovir	Plasma	LC-MS/MS	10 ng/mL 4 ng/mL	Active
Enalapril	Plasma	LC-MS/MS	0.5 ng/mL	Active
Entecavir	Plasma	LC-MS/MS	0.1 ng/mL	Active
Epinephrine	Plasma Urine	HPLC/ED	0.2 ng/mL	Active
Eplerenone	Plasma	LC-MS/MS	25 ng/mL	Active
Estradiol (Unconjugated) / Estrone (Unconjugated)	Plasma	LC-MS/MS	3 pg/ml / 8 pg/ml	Active
Estrone (Totall)	Plasma	LC-MS/MS	0.1 ng/mL	Pre-Active
Eremomycin	Plasma	LC-MS/MS	50 ng/mL	Active
Esomeprazole	Plasma	LC-MS/MS	1 ng/mL	Active
Ethinyl Estradiol	Plasma	LC-MS/MS	3 pg/ml	Active
Ethyl lysine	Plasma	LC-MS/MS	20 ng/mL	Active
Etoricoxib	Plasma	LC-MS/MS	10 ng/mL	Active
Ezetimibe / Ezetimibe Glucuronide	Plasma	LC-MS/MS	0.1 ng/mL 1 ng/mL	Active
Febuxostat	Plasma	LC-MS/MS	50 ng/mL	Active
Fexofenadine	Plasma	LC-MS/MS	1 ng/mL	Active
Finasteride	Plasma	LC-MS/MS	0.5 ng/mL	Active
Fingolimod ( FTY720 )/ Fingolimod phosphate ( FTY720-P)	Blood	LC-MS/MS	50 pg/mL 70 pg/mL	Active
Fingolimod ( FTY720 )	Blood	LC-MS/MS	20 pg/mL	Active
Fosfomycin	Plasma	LC-MS/MS	1 mkg/ml	Active
Furazidin	Plasma	LC-MS/MS	-	Development
Gliclazide	Plasma	LC-MS/MS	20 ng/mL	Active
Homovanillic acid	Urine	HPLC/ED	10 ng/mL	Active
Ibandronic Acid	Plasma	LC-MS/MS	0.8 ng/mL	Active
Ibuprofen	Plasma	LC-MS/MS	200 ng/mL	Active
Imatinib	Plasma	LC-MS/MS	5 ng/mL	Active
Inosine / Methionine / Nicotinamide	Plasma	LC-MS/MS	1/3 mkg/ml 200 ng/ml	Active
Irbesartan	Plasma	LC-MS/MS	5 ng/mL	Active
Itopride	Plasma	LC-MS/MS	5 ng/mL	Active
Ketorolac	Plasma	LC-MS/MS	10 ng/mL	Active
Kynurenine	Plasma	HPLC/UV	10 ng/mL	Inactive
Kynurenic acid	Plasma	HPLC/UV	20 ng/mL	Inactive
Lamotrigine	Plasma	LC-MS/MS	10 ng/mL	Active
Lenalidomide	Plasma	LC-MS/MS	1 ng/mL	Active
Lercanidipine	Plasma	LC-MS/MS	50 pg/ml	Active
Levetiracetam	Plasma	LC-MS/MS	200 ng/mL	Active
Levofloxacin	Plasma	LC-MS/MS	250 ng/mL	Active
Levonorgestrel	Plasma	LC-MS/MS	0.1 ng/mL	Active
Lidocaine	Plasma	LC-MS/MS	1 ng/mL	Active
Linezolid	Plasma	LC-MS/MS	100 ng/mL	Active
Lipoic acid	Plasma	LC-MS/MS	5 ng/mL	Active
Lisinopril	Plasma	LC-MS/MS	5 ng/mL	Active
Losartan	Plasma	LC-MS/MS	5 ng/mL	Active
Losartan / Hydrochlorothiazide	Plasma	LC-MS/MS	1.5 ng/mL 1 ng/mL	Active
Marcaine	Plasma Tissue	LC-MS/MS HPLC/UV	1 ng/mL	Active
Mebeverin Acid/ o-desmethyl mebeverine acid	Plasma	LC-MS/MS	1 ng/mL 5 ng/mL	Active
Melatonin	Plasma	LC-MS/MS	10 pg/ml	Active
Meloxicam	Plasma	LC-MS/MS	10 ng/mL	Active
Melphalan	Plasma Tissue	LC-MS/MS	0.3 ng/mL	Active
Memantine	Plasma	LC-MS/MS	0.3 ng/mL	Active
Metanephrine	Urine	HPLC/ED	10 ng/mL	Active
Metformin / Sitagliptin	Plasma	LC-MS/MS	10 ng/mL 1 ng/mL	Active
Metoprolol	Plasma	LC-MS/MS	0.1 ng/mL	Active
Mexidol (Emoxypine)	Plasma	LC-MS/MS	0.5 ng/mL	Active
Mildronate	Plasma	LC-MS/MS	50 ng/mL	Active
4-Methylaminoantipyrine (Metamizole metabolite)	Plasma	LC-MS/MS	100 ng/mL	Active
Mitomicyn C	Plasma Tissue	LC-MS/MS	0.6 ng/mL	Active
Mitotane	Plasma	HPLC-UV	0.75 mkg/mL	Active
Mitoxantrone	Plasma Tissue	LC-MS/MS	5 ng/mL	Inactive
Monomethyl fumarate	Plasma	LC-MS/MS	10 ng/mL	Active
Montelukast/ Cetirizine	Plasma	LC-MS/MS	10 ng/mL 1 ng/mL	Active
Moxifloxacin	Plasma	LC-MS/MS	2 ng/mL	Active
Moxonidine	Plasma	LC-MS/MS	50 pg/mL	Active
Naftidrofuryl	Plasma	LC-MS/MS	10 ng/mL	Active
Naloxone	Plasma	LC-MS/MS	80 pg/mL	Active
Naltrexone	Plasma	LC-MS/MS	150 pg/mL	Active
Nevirapine	Plasma	LC-MS/MS	80 ng/mL	Active
Nifedipine/ Lidocaine	Plasma	LC-MS/MS	0.5 ng/mL 1 ng/mL	Active
Nilotinib	Plasma	LC-MS/MS	2 ng/mL	Active
Nimesulide	Plasma	LC-MS/MS	20 ng/mL	Active
Nitisinone	Plasma	LC-MS/MS	5 ng/mL	Active
Norepinephrine	Plasma Urine	HPLC/ED	0.2 ng/mL	Active
Normetanephrine	Urine	HPLC/ED	5 ng/mL	Active
Oxybutynin	Plasma Tissue	LC-MS/MS	0.5 ng/mL	Active
Oseltamivir carboxylate	Plasma	LC-MS/MS	2 ng/mL	Active
PAS (4-Aminosalicylic acid)	Plasma	LC-MS/MS	50 ng/mL	Active
Pazopanib	Plasma	LC-MS/MS	300 ng/mL	Active
Penciclovir	Plasma	LC-MS/MS	50 ng/mL	Active
Perindopril / Perindoprilat	Plasma	LC-MS/MS	0.4 ng/mL 0.4 ng/mL	Active
Perindoprilat / Indapamide	Plasma	LC-MS/MS	0.4 ng/mL 0.3 ng/mL	Active
Phenibut	Plasma	LC-MS/MS	10 ng/mL	Active
Phenylephrine	Plasma	LC-MS/MS	30 pg/ml	Active
Pirfenidone	Plasma	LC-MS/MS	5 ng/mL	Active
Pregabalin	Plasma	LC-MS/MS	50 ng/mL	Active
Rabeprazole	Plasma	LC-MS/MS	2 ng/mL	Active
Ranolazine	Plasma	LC-MS/MS	5 ng/mL	Active
Ramipril / Ramiprilat	Plasma	LC-MS/MS	0.2 ng/mL 0.5 ng/mL	Active
Ritonavir	Plasma	LC-MS/MS	5 ng/mL	Active
Rivaroxaban	Plasma	LC-MS/MS	0.5 ng/mL	Active
Rhein (Diacerein)	Plasma	HPLC/UV	100 ng/mL	Active
Ropinipole	Plasma	LC-MS/MS	10 pg/mL	Active
Rosuvastatin	Plasma	LC-MS/MS	0.2 ng/mL	Active
Salbutamol	Plasma	LC-MS/MS	0.2 ng/mL	Active
Serotonin	Plasma	HPLC/UV	10 ng/mL	Active
Sildenafil	Plasma	LC- MS/MS	5 ng/mL	Active
Sildenafil	Plasma	LC-MS/MS	1 ng/mL	Active
Sitagliptin	Plasma	LC-MS/MS	1 ng/mL	Active
Solifenacin	Plasma	LC-MS/MS	0.5 ng/mL	Active
Spironolactone / Canrenone	Plasma	LC-MS/MS	2 ng/mL 2 ng/mL	Active
Stavudine	Plasma	LC-MS/MS	30 ng/mL	Active
Succinic acid	Plasma	LC-MS/MS	0.1 µg/mL	Active
Sunitinib	Plasma	LC-MS/MS	-	Development
Tadalafil	Plasma	LC-MS/MS	5 ng/ml	Active
Tacrolimus	Blood	LC-MS/MS	0.5 ng/mL 0.1 ng/mL	Active Active
Tamsulosin	Plasma	LC-MS/MS	0.1 ng/mL	Active
Telmisartan	Plasma	LC-MS/MS	3 ng/mL	Active
Telmisartan/ Hydrochlorothiazide	Plasma	LC-MS/MS	3 ng/mL 1 ng/mL	Active
Tenofovir	Plasma	LC-MS/MS	4 ng/mL	Active
Terbinafine	Plasma	LC-MS/MS	1 ng/mL	Active
Terizidone	Plasma	LC-MS/MS	0.3 µg/mL	Active
Thyroxine (T4)	Serum	LC-MS/MS	30 ng/mL	Active
Ticagrelor / AR-C124910XX	Plasma	LC-MS/MS	5 ng/mL/ 2.5 ng/mL	Active
Tilorone	Plasma	LC-MS/MS	0.5 ng/mL	Active
Tolperisone	Plasma	LC-MS/MS	0.5 ng/mL	Active
Troventol	Plasma	LC-MS/MS	35 pg/mL	Active
Troxerutin	Plasma	LC-MS/MS	20 pg/ml	Active
Tryptophan	Plasma	HPLC/UV	10 ng/mL	Active
Quetiapine	Plasma	LC-MS/MS	1 ng/mL	Active
Ursodeoxycholic acid	Plasma	LC-MS/MS	50 ng/mL	Active
Valsartan	Plasma	LC-MS/MS	10 ng/mL	Active
Vanillyl mandelic acid	Urine	HPLC/UV	10 ng/mL	Active
Vildagliptin / Metformin	Plasma	LC-MS/MS	2 ng/mL 5 ng/mL	Active
Vinblastine	Plasma Tissue	LC-MS/MS HPLC/UV	1 ng/mL	Active
Voriconazole	Plasma	LC-MS/MS	20 ng/mL	Active
Warfarin	Plasma	LC-MS/MS	5 ng/mL	Active
Zidovudine	Plasma	LC-MS/MS	40 ng/mL	Active
Zidovudine / Lamivudine	Plasma	LC-MS/MS	30 ng/mL 25 ng/mL	Active